Immunofluorescence staining of K562 cells with CSB-RA019386A780phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Immunoprecipitating Phospho-RB1 in Hela whole cell lysate
Lane 1: Rabbit control IgG(1ug)instead of CSB-RA019386A780phHU in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-RA019386A780phHU(3ug)+ Hela whole cell lysate(1mg)
Lane 3: Hela whole cell lysate (20ug)